Preparation of nucleic acid templates for solid phase amplification
US8168388B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 24, 2006 |
| Grant date | May 1, 2012 |
| Priority date | — |
| Expiry date | Mar 26, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.