Process for chromosomal integration and DNA sequence replacement in Clostridia
US8168434B2 · kind B2 · utility
2Cited by
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20Claims
0Family size
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Key dates
| Filing date | Apr 18, 2007 |
| Grant date | May 1, 2012 |
| Priority date | — |
| Expiry date | Apr 4, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/74
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.