Systems and methods for protein production
US8173394B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 6, 2009 |
| Grant date | May 8, 2012 |
| Priority date | — |
| Expiry date | Apr 6, 2029 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2217/075
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XB1- or ATF6-mediated UPR pathway.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.