Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher eucaryotic cells using electrical current

US8173416B2 · kind B2 · utility

Assignee

• Lonza Cologne GmbH · DE

Inventors

• Herbert Muller-Hartmann
• Gudula Riemen · Langenfeld, DE
• Kirsten Rothmann-Cosic · Berlin, DE
• Corinna Thiel · Cologne, DE
• Ludger Altrogge · Pulheim, DE
• Meike Weigel
• Rainer Christine
• Elke Lorbach · Cologne, DE
• Juliana Helfrich · Glauchau, DE
• Heike Wessendorf
• Gregor Siebenkotten · Frechen, DE

Key dates

• Filing date: Apr 23, 2002
• Grant date: May 8, 2012
• Expiry date: Sep 28, 2027

Classification

• Technology area (CPC H): Electricity
• CPC primary: H03K3/57
• WIPO field: Medical technology
• WIPO sector: Instruments

Abstract

The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.