Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays

US8178359B2 · kind B2 · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Thoams Krahn · Hagen, DE
• Wolfgang Paffhausen · Leverkusen, DE
• Andreas Schade · Lengede, DE
• Martin Bechem · Wuppertal, DE
• Delf Schmidt · Wuppertal, DE

Key dates

• Filing date: Sep 28, 2001
• Grant date: May 15, 2012
• Expiry date: Dec 22, 2022

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/13
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A process for the quantitative optical analysis of fluorescently labeled biological cells involves contacting a cell layer on a transparent support at the bottom of a reaction vessel with a solution containing the fluorescent dye. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved.