Patent · US Active

Homogeneous luminescence bioassay

US8182988B2 · kind B2 · utility

4Cited by

1References

25Claims

0Family size

Assignee

Hidex Oy · FI

Inventors

Tero Soukka · Turku, FI
Urpo Juhani Lamminmaki · Ilmarinen, FI

Key dates

Filing date	Feb 26, 2008
Grant date	May 22, 2012
Priority date	—
Expiry date	Jan 15, 2029

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/542
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A homogenous bioassay including i) a first group containing a short lifetime fluorescent acceptor, and ii) a second group containing a quencher, with the first and second groups linked by at least a first linkage. The bioassay measures the acceptor's fluorescence increase resulting from cleavage of the first linkage and also includes iii) a third group containing a donor for energy transfer to the acceptor, where the donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound. A conformational or terminal epitope is created on the first group through linkage cleavage, and the third group includes a binder with affinity for this epitope. The acceptor's fluorescence is caused by exciting the donor. Also disclosed are bioassay kits for this method.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.