Recombinant human hepatitis C virus-like particle and method for producing the same

US8183044B2 · kind B2 · utility

Assignees

• JAPAN as represented by DIRECTOR GENERAL of National Institute of Infectious Diseases · JP
• TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE · JP
• TORAY INDUSTRIES, INC. · JP

Inventors

• Jun Tanabe · Yamaguchi, JP
• Saburo Sone · Yokohama, JP
• Takaji Wakita · Tokyo, JP
• Koji Ishii · Tokyo, JP
• Ryosuke Suzuki · Anjo, JP
• Tetsuro Suzuki · Fuji, JP
• Tatsuo Miyamura · Tokyo, JP

Key dates

• Filing date: Sep 29, 2006
• Grant date: May 22, 2012
• Expiry date: Nov 6, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/24252
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for producing a recombinant hepatitis C virus-like particle comprising the steps of introducing into (i) a cell in which an RNA replicon comprising a nucleotide sequence comprising the 5′ untranslated region, the nucleotide sequence coding for the NS3, NS4A, NS4B, NS5A, and NS5B proteins, and the 3′ untranslated region of a genome RNA derived from a hepatitis C virus strain autonomously replicates, (ii) a vector expressing the Core, E1, E2, and p7 proteins derived from a hepatitis C virus strain that is the same as or different from that as defined in the above (i), culturing the cell, and recovering the produced virus-like particle, and a recombinant hepatitis C virus particle produced by this method.