Method of determining initial concentration of nucleic acid in sample using real-time amplification data
US8185324B2 · kind B2 · utility
2Cited by
1References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 28, 2008 |
| Grant date | May 22, 2012 |
| Priority date | — |
| Expiry date | Aug 31, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6851
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method of determining an initial concentration of a target nucleic acid within a sample using real-time nucleic acid amplification data. Amplification efficiencies of the target nucleic acid with respect to amplification time are obtained from signals of amplified products, and an amplification efficiency function with respect to amplification time is formulated employing the amplification efficiencies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.