Versatile acylation catalytic RNAs and uses thereof

US8188260B2 · kind B2 · utility

Assignee

• THE UNIVERSITY OF TOKYO · JP

Inventors

• Hiroaki Suga · Tokyo, JP
• Hiroshi Murakami · Funabashi, JP

Key dates

• Filing date: Dec 5, 2006
• Grant date: May 29, 2012
• Expiry date: Jan 8, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/34
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An object of the present invention is to provide novel ribozyme systems capable of catalyzing tRNA acetylation using various carboxylic acids as acyl donors and uses thereof. Disclosed is a ribozyme catalyzing tRNA acetylation having a structure consisting of the RNA sequence represented by: (formula 1) P1-Z1Z2Z3Z4(N1)1(N1)2 . . . (N1)p-P2-(N2)1(N2)2 . . . (N2)q Y1Y2Y3-(N3)1(N3)2-N4GGN wherein (N1)1-(N1)p each independently represent any monoribonucleotide of U, C, A, and G; p represents 3 or 4; (N2)1-(N2)q each independently represent any monoribonucleotide of U, C, A, and G; q represents 5 or 6; (N3)1-(N3)2 each independently represent any monoribonucleotide of U, C, A, and G; N4 represents any monoribonucleotide of U, C, A, and G; Z1-Z4 each independently represent C or G; Y1-Y3 each independently represent C or G; N represents a monoribonucleotide complementary to A or G; and P1 and P2 represent a domain consisting of any RNA sequence capable of having a stem-loop structure.