Patent · US Active

Methods for assessing molecular expression of subcellular molecules

US8189884B2 · kind B2 · utility

2Cited by
1References
24Claims
0Family size

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Inventors

Key dates

Filing dateMar 26, 2008
Grant dateMay 29, 2012
Priority date
Expiry dateMar 30, 2031

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG06T2207/30072
  • WIPO fieldComputer technology
  • WIPO sectorElectrical engineering

Abstract

The present techniques provide fully automated methods for quantifying the location, strength and percent of expressed target molecules or other biological markers in immunohistochemically stained biological samples. The samples may be automatically segmented, for example into subcellular compartments, from images of compartmental markers. Then, the distribution of a target molecule on each of these compartments is calculated that includes the percentage and strength of expression. This is different than existing intensity or ratio based methods where abundant low expression levels are indistinguishable from scarce high expression levels.

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