Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase
US8192932B2 · kind B2 · utility
1Cited by
11References
19Claims
0Family size
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Key dates
| Filing date | Feb 12, 2010 |
| Grant date | Jun 5, 2012 |
| Priority date | — |
| Expiry date | Feb 12, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y599/01002
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5′ end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5′ end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.