Promoter and plasmid system for genetic engineering

US8192959B2 · kind B2 · utility

Assignees

• GENENCOR INTERNATIONAL, INC. · US
• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Mark S. Payne · Wilmington, US
• Stephen Picataggio · Rockland, US
• Amy Kuang-Hua Hsu · Loomis, US
• Ramesh Nair · Ankeny, US
• Fernando Valle · Palo Alto, US
• Philippe Soucaille · Deyme, FR
• Donald E. Trimbur · Palo Alto, US

Key dates

• Filing date: Apr 22, 2003
• Grant date: Jun 5, 2012
• Expiry date: Jan 27, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P7/18
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.