Sequential protein isolation and purification schemes by affinity chromatography
US8198407B1 · kind B1 · utility
22Cited by
19References
14Claims
0Family size
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Key dates
| Filing date | Aug 19, 2005 |
| Grant date | Jun 12, 2012 |
| Priority date | — |
| Expiry date | Oct 4, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/775
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention discloses methods for sequential protein isolation and purification from a biological sample by affinity chromatography. Affinity chromatography is conducted using ligands or ligand support complexes that selectively and specifically bind to proteins in the biological sample. The ligands or ligand support complexes were contacted sequentially in a predetermined order with the biological sample to allow each ligand or ligand-support complex to sequentially bind a protein from the biological sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.