Enzyme assays for a droplet actuator
US8202686B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 5, 2009 |
| Grant date | Jun 19, 2012 |
| Priority date | — |
| Expiry date | Mar 16, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/042
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a microfluidic platform and methods of using the platform for conducting enzyme assays using a droplet actuator. The enzyme assays of the invention are useful for, among other things, identifying and/or characterizing disorders resulting from conditions in which enzymes are defective or are produced in inappropriate amounts. Enzyme assays of the invention may, for example, be used to detect altered activity of a particular enzyme in a sample, which may serve as an indicator of a particular disease. Altered activity may, for example, be caused by conditions which result in the increased or reduced production of a certain enzyme or its substrate and/or conditions which result in defective enzymes and/or substrates exhibiting increased or decreased effectiveness relative to corresponding normal enzymes and/or substrates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.