Patent · US Active

Uniform fragmentation of DNA using binding proteins

US8202691B2 · kind B2 · utility

8Cited by

9References

19Claims

0Family size

Assignee

Illumina, Inc. · US

Inventors

Frank J. Steemers · Encinitas, US
Jonathan Mark Boutell · Stanford-le-Hope, GB

Key dates

Filing date	Jan 22, 2009
Grant date	Jun 19, 2012
Priority date	—
Expiry date	Aug 28, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6869
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention provides a method for preparing and analysing a population of fragmented polynucleotide sequences having a substantially uniform size. The method can include steps of (a) binding at least one protection molecule to at least one polynucleotide sequence; (b) cleaving the at least one polynucleotide sequence to generate a plurality of polynucleotide fragment sequences of substantially uniform size; (c) amplifying the polynucleotide fragments; and (d) determining a sequence characteristic of a plurality of the polynucleotide fragments.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.