Enzymatic time-resolved luminescent assay for nucleic acids quantitation
US8206905B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Apr 26, 2008 |
| Grant date | Jun 26, 2012 |
| Priority date | — |
| Expiry date | May 16, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The objects of the present invention are to provide a new technology platform for quantitation number of copies of nucleic acid molecules of interest by lumonogenic (i.e., enzymatic luminescence) detection. The detection approach of the method of present invention is essentially employing time-resolved approach, e.g., based on detection transient parameters of luminescent signal. The various disclosed embodiments allow DNA and RNA quantification in a broad dynamic range; can be used for detection of DNA, as well as long (mRNA) and short (microRNA) RNA targets. The present invention provides methods, instruments, and kits for fast and highly sensitive identification and measurements of nucleic acids in various life science and biomedical applications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.