Methods for high fidelity production of long nucleic acid molecules

US8206952B2 · kind B2 · utility

Assignee

• Massachusetts Institute of Technology · US

Inventors

• Peter A. Carr · Medford, US
• Brian Yichiun Chow · Cambridge, US
• Joseph M. Jacobson · Newton, US
• David Mosley · Philadelphia, US
• Christopher J. Emig · Napa, US

Key dates

• Filing date: Feb 1, 2011
• Grant date: Jun 26, 2012
• Expiry date: Feb 1, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6848
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In a method for synthesizing a long nucleic acid molecule, a first immobilized nucleic acid has a first 5′ region and a first 3′ region and a second immobilized nucleic acid has a second 5′ region and a second 3′ region. The second 3′ region and the first 5′ region have identical nucleic acid sequences. An oligonucleotide is hybridized to the first 3′ region, extending the hybridized oligonucleotide and producing a first extension product having a 3′ region that is complementary to the first 5′ region. The 3′ region of the first extension product is hybridized to the second 3′ region, extending the 3′ region of the first extension product and producing a synthesized nucleic acid molecule having a 3′ region that is complementary to the second 5′ region, wherein the synthesized nucleic acid molecule has a sequence complementary to the first and second 3′ and 5′ regions.