Modified Luciola cruciata luciferase protein
US8206961B2 · kind B2 · utility
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Key dates
| Filing date | May 24, 2010 |
| Grant date | Jun 26, 2012 |
| Priority date | — |
| Expiry date | May 24, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/107
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
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