Protease screening methods and proteases identified thereby
US8211428B2 · kind B2 · utility
23Cited by
67References
59Claims
0Family size
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Key dates
| Filing date | Jul 5, 2007 |
| Grant date | Jul 3, 2012 |
| Priority date | — |
| Expiry date | Oct 23, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/42
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods for identifying modified proteases with modified substrate specificity or other properties are provided. The methods screen candidate and modified proteases by contacting them with a substrate, such as a serpin, an alpha macroglobulins or a p35 family protein or modified serpins and modified p35 family members or modified alpha macroglobulins, that, upon cleavage of the substrate, traps the protease by forming a stable complex. Also provided are modified proteases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.