Apparatus and methods for osmotically shocking cells
US8211668B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 11, 2008 |
| Grant date | Jul 3, 2012 |
| Priority date | — |
| Expiry date | Aug 18, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/78
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of preparing a recombinant polypeptide of interest includes fermenting a host cell being transformed with a recombinant expression system capable of bringing about secretion of a polypeptide of interest into the periplasm of said host cell. The polypeptide of interest is extracted from the periplasm by applying a continuous osmotic shock to the host cells contained in a fermentation medium. An apparatus for osmotically shocking cells includes a first reservoir containing cells in a first solution and a second reservoir containing a second solution, the first solution having a higher osmolarity than the second solution. A method for osmotically shocking cells using the first and second solutions is also disclosed. Also disclosed is a method of isolating a recombinant polypeptide of interest from a cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.