Plasmid, transformants and process for production of 3- carboxymuconolactone
US8211683B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 10, 2007 |
| Grant date | Jul 3, 2012 |
| Priority date | — |
| Expiry date | Nov 3, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P17/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
There is provided a process for industrial production of simple 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone from low molecular mixtures derived from plant components such as vanillin, vanillic acid and protocatechuic acid, via a multistage enzyme reaction. A recombinant plasmid containing a vanillate demethylase gene (vanAB genes), benzaldehyde dehydrogenase gene (ligV gene) and protocatechuate 3,4-dioxygenase gene (pcaHG genes); transformants incorporating the plasmid; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of vanillin, vanillic acid, protocatechuic acid or a mixture of two or more thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.