Patent · US Active

I-DmoI derivatives with enhanced activity at 37° C and use thereof

US8211685B2 · kind B2 · utility

4Cited by
0References
22Claims
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Assignee

Inventors

Key dates

Filing dateApr 27, 2005
Grant dateJul 3, 2012
Priority date—
Expiry dateMar 18, 2027

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N9/22
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

I-DmoI derivatives with enhanced cleavage activity at 37° C., said mutant comprising a sequence of a mutant of a I-DmoI endonuclease or a chimeric 5 derivative thereof including at least the first I-DmoIdomain, said sequence comprising the sub-situation of at least: (i) one of the residues in positions 4, 20, 49, 52, 92, 94 and/or 95 of said first I-DmoIdomain, and/or (ii) one of the residues in positions 101, 102, and/or 109 of the linker or the beginning of the second domain of I-DmoI, if present. 10 Polynucleotide encoding said derivatives, cell, animal or plant comprising said polynucleotide and use thereof for isolating meganucleases with new DNA target specificity.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.