Method and an apparatus for localization of single dye molecules in the fluorescent microscopy

US8212866B2 · kind B2 · utility

Assignee

• Ruprecht-Karls-Universitat Heidelberg Kirchhoff-Institut fur Physik · DE

Inventors

• Paul Lemmer · Deggingen, DE
• Christoph Cremer · Heidelberg, DE
• David Baddeley · Heidelberg, DE
• Heinz Eipel · Bensheim, DE

Key dates

• Filing date: Mar 16, 2009
• Grant date: Jul 3, 2012
• Expiry date: Dec 20, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6441
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method and apparatus are provided for obtaining a sub-resolution spatial information of a sample labeled with at least one type fluorescent label. The sub-resolution spatial information has localization information about the positions of fluorescent molecules of the at least one type fluorescent label in at least one spatial direction. The method acquires localization image data by employing fluorescence localization microscopy. The acquired localization image data is processed to obtain the localization information about the positions of fluorescent molecules of the at least one type fluorescent label in at least one spatial direction. The step of processing includes determining in each of the detected images of the series the positions of the barycenters of the detected fluorescence emission distributions from the single fluorescent molecules of the one or more fluorescent labels in at least one spatial direction.