Characterization of N-glycan mixtures by nuclear magnetic resonance

US8216851B2 · kind B2 · utility

Assignee

• MOMENTA PHARMACEUTICALS, INC. · US

Inventors

• Ian Christopher Parsons · Belmont, US
• Jonathan C. Lansing · Reading, US
• Daniela Beccati · Boston, US
• Scott Bailey · Round Rock, US
• Carlos J. Bosques · Arlington, US

Key dates

• Filing date: Apr 15, 2008
• Grant date: Jul 10, 2012
• Expiry date: Jul 5, 2028

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/24
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure provides nuclear magnetic resonance (NMR) methods for characterizing mixtures of N-linked glycans. Without limitation, methods of the present disclosure may be useful in characterizing monosaccharide composition, branching, fucosylation, sulfation, phosphorylation, sialylation linkages, presence of impurities and/or efficiency of a labeling procedure (e.g., labeling with a fluorophore such as 2-AB). In certain embodiments, the methods can be used quantitatively. In certain embodiments, the methods can be combined with enzymatic digestion to further characterize glycan mixtures.