Microscopic imaging techniques
US8217992B2 · kind B2 · utility
10Cited by
31References
27Claims
0Family size
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Key dates
| Filing date | Jan 11, 2008 |
| Grant date | Jul 10, 2012 |
| Priority date | — |
| Expiry date | May 12, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B27/58
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
A method of performing 3D photoactivation microscope imaging includes providing a sample having a plurality of probes, each of the plurality of probes including a photo-activatable material. Probes from the plurality of probes are activated to form a sparse subset of probes, the sparse subset of probes having probes that are spatially separated by at least a microscope resolution. The sample is illuminated with a readout light source, and light emitted from activated probes is detected. Based on the light emission detected from the activated probes, localized three-dimensional positions of the activated probes are obtained.
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