Use of conjugates with linkers cleavable by photodissociation or fragmentation for mass spectrometry analysis of tissue sections

US8221972B2 · kind B2 · utility

Assignees

• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR
• UNIVERSITE DES SCIENCES ET TECHNOLOGIES DE LILLE 1 · FR
• EUROGENTEC S.A. · FR

Inventors

• Rémi Lemaire · Ormesson-sur-Marne, FR
• Isabelle Fournier · Bourghelles, FR
• Michel Salzet · Bourghelles, FR
• Michel Dechamps · Verviers, BE
• Jean-Claude Edmond Tabet · Morangis, FR
• Gottfried Proess · Bütgenbach, BE
• Ivo Rudloff · Essen, DE
• Marc Lemaitre · Cincinnati, US

Key dates

• Filing date: Jun 7, 2006
• Grant date: Jul 17, 2012
• Expiry date: Oct 10, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/24
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention concerns a method for determining at least one target molecule map in a tissue section, using at least one (A-X)n-B conjugate, wherein A is a tag molecule of known molecular weight, X is a linker that is cleaved during sample desorption/ionization, n is an integer of at least 1, and B is a binding molecule that binds specifically to said target molecule. When using MALDI mass spectrometry, said linker molecule X may be cleaved by photodissociation during sample laser irradiation if photocleavable at the wavelength of said MALDI laser. Alternatively, when using UV-MALDI, IR-MALDI, SIMS or DESI mass spectrometry, said linker molecule X may be cleaved by fragmentation during sample desorption/ionization.