Protein production

US8221999B2 · kind B2 · utility

Assignee

• BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG · DE

Inventors

• Hitto Kaufmann · Ulm, DE
• Lore Florin · Biberach, DE
• Eric Becker · Biberach an der Riß, DE
• Monilola Olayioye · Stuttgart, DE
• Angelika Hausser · Stuttgart, DE
• Tim Fugmann · Zürich, CH

Key dates

• Filing date: Feb 29, 2008
• Grant date: Jul 17, 2012
• Expiry date: Apr 5, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/036
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport.