Patent · US Active

Ketol-acid reductoisomerase using NADH

US8222017B2 · kind B2 · utility

37Cited by

2References

14Claims

0Family size

Assignee

Butamax Advanced Biofuels LLC · US

Inventors

Yougen Li · Pennington, US
Der-Ing Liao · Wilmington, US
Mark J. Nelson · Newark, US
Daniel P. Okeefe · Swarthmore, US
Jean-Francois Tomb · Wilmington, US

Key dates

Filing date	Nov 15, 2011
Grant date	Jul 17, 2012
Priority date	—
Expiry date	Nov 15, 2031

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY02E50/10
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.