Integrated nucleic acid assays

US8222023B2 · kind B2 · utility

Assignee

• MICRONICS, INC. · US

Inventors

• C. Frederick Battrell · Redmond, US
• John Gerdes · Denver, US
• John R. Williford · Sammamish, US
• Denise Maxine Hoekstra · Monroe, US
• Wayne L. Breidford · Seattle, US
• Stephen Mordue · Kirkland, US
• John Clemmens · Redmond, US
• Melud Nabavi · Seattle, US
• Mark Stamatios Kokoris · Bothell, US

Key dates

• Filing date: Sep 10, 2008
• Grant date: Jul 17, 2012
• Expiry date: Aug 6, 2029

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/0655
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.