Patent · US Active

Methods of optimal purification of nucleic acids and kit for use in performing such methods

US8222397B2 · kind B2 · utility

4Cited by
130References
8Claims
0Family size

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Inventor

Key dates

Filing dateAug 28, 2009
Grant dateJul 17, 2012
Priority date—
Expiry dateMay 11, 2030

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/107497
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method and kit which allow the use of a discrete amount of a binding matrix to first purify nucleic acids from a medium under a first set of binding conditions wherein the amount of nucleic acid bound to the binding matrix is essentially independent of the amount of surface area of the definable amount of the binding matrix, followed by a second purification step wherein the nucleic acids are bound to a discrete amount of binding matrix under a second set of binding conditions wherein the amount of nucleic acid bound to the binding matrix is essentially dependent on the amount of surface area of the definable amount of the binding matrix, thus providing a discrete quantity of nucleic acid.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.