Bactobolin analog and synthesis method thereof

US8222434B1 · kind B1 · utility

Assignees

• University of Washington through its Center for Commercialization · US
• President and Fellows of Harvard College · US

Inventors

• E. Peter Greenberg · Iowa City, US
• Josephine R. Chandler · Seattle, US
• Breck Duerkop · Dallas, US
• Patricia Lima · Duque de Caxias, BR
• Joshua Alan Van Dyke Blodgett · Webster Groves, US
• Jon Clardy · Ithaca, US
• Mohammad R. Seyedsayamdost · Princeton, US

Key dates

• Filing date: Apr 5, 2010
• Grant date: Jul 17, 2012
• Expiry date: Jun 11, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07D311/76
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The present inventions are directed to a novel bactobolin analog bactobolin D. The present inventions also are directed to a method of producing a composition comprising at least one bactobolin analog using a bacteria strain comprising a bacterial cell comprising the biosynthetic locus of the bactobolin analog in Burkholderia thailandensis (bta cluster) or a homolog structure (at least 95% sequence identity) thereof, and further isolation and purification of the bactobolin analog. For example, the bacterial strain can be a wild type bacterial strain such as a Burkholderia strain comprising a bta cluster (e.g. Burkholderia thailandensis (e.g. E264, Bt4, and TXDOH) and Burkholderia pseudomallei (e.g. K96243, 1106a, and 1106b).