Method for positioning biological samples in a microscopic arrangement

US8228499B2 · kind B2 · utility

Assignee

• Carl Zeiss Micromanaging GmbH · DE

Inventors

• Helmut Lippert · Jena, DE
• Benno Radt · Jena, DE
• Christian Dietrich · Jena, DE
• Christopher Power · Jena, DE

Key dates

• Filing date: Sep 18, 2008
• Grant date: Jul 24, 2012
• Expiry date: Feb 3, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/34
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices are proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation.