Comparative genomic hybridization assays using immobilized oligonucleotide features and compositions for practicing the same

US8232055B2 · kind B2 · utility

Assignee

• AGILENT TECHNOLOGIES, INC. · US

Inventors

• Laurakay Bruhn · Palo Alto, US
• Alicia Scheffer · Redwood City, US
• Michael Barrett · Georgetown, US
• Douglas Amorese · Los Altos, US
• Stephen S. Laderman · Menlo Park, US

Key dates

• Filing date: Dec 22, 2003
• Grant date: Jul 31, 2012
• Expiry date: Sep 3, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6809
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Comparative genomic hybridization assays and compositions for use in practicing the same are provided. A characteristic of the subject comparative genomic hybridization assays is that solid support immobilized oligonucleotide feature elements, e.g., in the form of an array, are employed. Specifically, at least first and second nucleic acid populations prepared from genomic templates are contacted with a plurality of distinct oligonucleotide feature elements immobilized on a solid support surface and the binding of the at least first and second populations is then evaluated. Also provided are kits for use in practicing the subject methods.