Growth medium for Clostridium histolyticum

US8236356B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Bernhard Suppmann · Weilheim, DE
• Werner Hoelke · Penzberg, DE
• Artur Hoffmann · Wolfratshausen, DE
• Thomas Marx · Penzberg, DE
• Kirsten Sonn · Penzberg, DE
• Johann-Peter Thalhofer · Weilheim, DE

Key dates

• Filing date: Jun 4, 2009
• Grant date: Aug 7, 2012
• Expiry date: Apr 8, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferably plant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.