Automatic focusing apparatus for use in a microscope in which fluorescence emitted from a cell is captured so as to acquire a cell image, and automatic focusing method therefor
US8237785B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 13, 2007 |
| Grant date | Aug 7, 2012 |
| Priority date | — |
| Expiry date | Jul 10, 2029 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH04N23/673
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In acquisition of an image of cells, a focal position is accurately set at highly active cells rather than focusing on dead cells. Provided is an automatic focusing apparatus (8) used in a microscope (1) that image captures fluorescence emitted from cells to acquire a cell image, the automatic focusing apparatus (8) including a setting unit (5) that sets a luminance range indicating a region where viable cells exist on the basis of a luminance distribution of the acquired cell image; and a focus-detecting unit that detects a focal position on the basis of a luminance of an image of nuclei of the cells within the luminance range set by the setting unit (5).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.