Cell free methods for detecting protein-ligand binding
US8241860B2 · kind B2 · utility
13Cited by
13References
24Claims
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Key dates
| Filing date | Nov 3, 2008 |
| Grant date | Aug 14, 2012 |
| Priority date | — |
| Expiry date | Nov 27, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6845
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided are rapid and sensitive cell-free assay methods for detecting and/or measuring specific bimolecular or higher order interactions via reassembly of a split monomeric reporter protein, and methods of detecting or identifying modulators of such interactions by the effect on the signal provided by the reassembled split reporter protein. This methodology is adaptable to protein-protein, protein-peptide, protein-nucleic acid, protein-methylated or nonmethylated nucleic acid and other small or large molecule ligands and binding proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.