Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof
US8241878B2 · kind B2 · utility
39Cited by
6References
15Claims
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Key dates
| Filing date | Nov 15, 2011 |
| Grant date | Aug 14, 2012 |
| Priority date | — |
| Expiry date | Nov 15, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.