Preparation of an artificial transcription factor comprising zinc finger protein and transcription factor of prokaryote, and a use thereof
US8242242B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 15, 2006 |
| Grant date | Aug 14, 2012 |
| Priority date | — |
| Expiry date | May 28, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to an artificial transcription factor which can artificially regulate gene expression of an E. coli, wherein the transcription factor comprising zinc finger proteins and transcription factors of prokaryote, and to be engineered E. coli using the same. Specifically, the artificial transcription factors comprising zinc finger domains and transcription factors in E. coli as effector domains are prepared and said artificial transcription library is introduced to E. coli to effectively and artificially regulate gene expression regardless of an activity of endogenous transcription factors in the E. coli and to induce E. coli having various desired phenotypes. Thus, only E. coli having the desired phenotypes useful for industries can be selected and used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.