Nucleic acid sequencing system and method using a subset of sites of a substrate
US8244479B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 25, 2009 |
| Grant date | Aug 14, 2012 |
| Priority date | — |
| Expiry date | Feb 2, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B25/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.