Method for increasing efficiency or specificity of hybridization between PNA probes immobilized on support and target nucleic acids
US8252533B2 · kind B2 · utility
6Cited by
3References
10Claims
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Key dates
| Filing date | Feb 22, 2008 |
| Grant date | Aug 28, 2012 |
| Priority date | — |
| Expiry date | Sep 27, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6834
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are a process for increasing efficiency or specificity of hybridization between peptide nucleic acid (PNA) probes immobilized on a support and target nucleic acids, which comprises the step of fragment the target nucleic acids to reduce the size of the target nucleic acids, or selectively degrading the target nucleic acids which mismatch with the PNA probes, in the hybridization reaction between the PNA probes supported on a support and the target nucleic acids; and a composition and a kit therefor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.