Patent · US Active

RNA interference tags

US8252535B2 · kind B2 · utility

31Cited by

0References

40Claims

0Family size

Assignee

QIAGEN GmbH · DE

Inventors

Wolfgang Biekle · Cologne, DE
Peter Hahn · Bensberg, DE

Key dates

Filing date	Apr 10, 2007
Grant date	Aug 28, 2012
Priority date	—
Expiry date	Apr 20, 2027

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2320/50
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a method for inhibiting the expression of a target gene in a eukaryotic cell. The method includes the following steps: a) providing at least one eukaryotic cell, the cell being capable of RNA interference, b) transfecting the eukaryotic cell with a composition that includes a genetic construct that includes an siRNA tag, and a target gene that forms a transcription unit together with the siRNA tag, and c) introducing at least one siRNA that is complementary to the siRNA tag of the transfected genetic construct to inhibit the expression of the target gene.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.