Nucleic acid encoding a cobalamin-dependent methionine synthase polypeptide

US8252555B2 · kind B2 · utility

Assignee

• EVONIK DEGUSSA GMBH · DE

Inventors

• Oskar Zelder · Speyer, DE
• Wolfgang Grabarse · Mannheim, DE
• Corinna Klopprogge · Mannheim, DE
• Hartwig Schröder · Nümbrecht, DE
• Stefan Haefner · Speyer, DE
• Anja Knietsch · Unna, DE
• Andrea Herold · Weinheim, DE

Key dates

• Filing date: Dec 21, 2007
• Grant date: Aug 28, 2012
• Expiry date: May 3, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/12
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to nucleotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof that are modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of such nucleotide sequences.