Patent · US Active

Bisulfite conversion of DNA

US8257950B2 · kind B2 · utility

21Cited by

3References

7Claims

0Family size

Assignee

EPIGENOMICS AG · DE

Inventors

Kurt Berlin · Stahnsdorf, DE
Matthias Ballhause · Berlin, DE
Karen Cardon · Albuquerque, US

Key dates

Filing date	Jun 13, 2011
Grant date	Sep 4, 2012
Priority date	—
Expiry date	Jun 13, 2031

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10T436/143333
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.