Luciferases and methods for making and using the same

US8258277B2 · kind B2 · utility

Assignees

• The Board of Trustees of the Leland Stanford Junior University · US
• The Regents of the University of California · US

Inventors

• Sanjiv S. Gambhir · Portola Valley, US
• Andreas M. Loening · Stanford, US
• Anna M. Wu · West Hollywood, US

Key dates

• Filing date: Mar 22, 2012
• Grant date: Sep 4, 2012
• Expiry date: Mar 22, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/66
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.