Mismatched end DNA ligase
US8263332B2 · kind B2 · utility
0Cited by
0References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 11, 2007 |
| Grant date | Sep 11, 2012 |
| Priority date | — |
| Expiry date | Jan 5, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2521/501
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A mismatched end DNA ligase is provided, which ligates two single strands to each other at a high efficiency, even if the other two single strands are not compatible. In one embodiment, the polypeptides of the ligase are Ku, Cernunnos, and XRCC4/Ligase4 (XL). This association can ligate DNA ends with a 3′ overhang to a recessed 5′ end, to a blunt end, or to a compatible end. In another embodiment, the proteins are Ku, Cernunnos, XRCC4/Ligase4 (XL) and DNA-PK.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.