Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US8263365B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 18, 2011 |
| Grant date | Sep 11, 2012 |
| Priority date | — |
| Expiry date | Jan 18, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/629
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.