Perifusion device
US8263389B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 30, 2010 |
| Grant date | Sep 11, 2012 |
| Priority date | — |
| Expiry date | Nov 22, 2030 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T137/87249
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A perifusion device includes at least one sample container for cells, the sample container having an inlet and an outlet. The container receives test liquid through the inlet and discharges the liquid through the outlet. A manifold having a plurality of liquid inlets, control valves, and liquid outlets can be provided so that the flow of liquids from source containers to the sample containers can be varied and controlled. A receptacle housing has a plurality of receptacles for receiving fluid from the outlet of the sample container. A drive is connected to the receptacle housing for moving the receptacle housing such that liquid samples are collected sequentially from the outlet of the sample containers. A programmable controller can be provided to control movement of the receptacle housing at predetermined times, and to record data identifying liquid samples in the receptacles. The test liquid includes at least one stimuli for the cells, which can be the presence, absence, or concentration of a compound in the liquid, or a physical property of the liquid such as temperature. The liquid collected in the receptacles is analyzed to determine the response of the cells to the stimuli.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.