Methods for detection of methyl-CpG dinucleotides
US8263742B2 · kind B2 · utility
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2References
7Claims
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Inventor
Key dates
| Filing date | Apr 12, 2011 |
| Grant date | Sep 11, 2012 |
| Priority date | — |
| Expiry date | Apr 12, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods for enriching methyl-CpG sequences from a DNA sample. The method makes use of conversion of cytosine residues to uracil under conditions in which methyl-cytosine residues are preserved. Additional methods of the invention enable to preservation of the context of me-CpG dinucleotides. The invention also provides a recombinant, full length and substantially pure McrA protein (rMcrA) for binding and isolation of DNA fragments containing the sequence 5′-CMeCpGG-3′. Methods for making and using the rMcrA protein, and derivatives thereof are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.