Method for combined sequential agent delivery and electroporation for cell structures and use thereof
US8268555B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 2009 |
| Grant date | Sep 18, 2012 |
| Priority date | — |
| Expiry date | Sep 22, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/1039
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed is a method for sequential delivery of agents to and/or into a cell structure, wherein an electrolyte-filled tube is provided together with a counter electrode, said tube is connected to a voltage or current generator, at least two agents are introduced in a discrete mode into the electrolyte solution contained in the tube, which is placed close to the cell structure, one agent at the time being transported through the tube to and/or into said cell structure in which a pore has been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. us of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.