Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
US8268582B2 · kind B2 · utility
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31Claims
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Key dates
| Filing date | Oct 22, 2004 |
| Grant date | Sep 18, 2012 |
| Priority date | — |
| Expiry date | Jun 19, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
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